Genomic DNA Isolation from Gram Positive Bacteria Kit
Genomic DNA Isolation from Gram Positive Bacteria Kit
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Introduction
The MBST DNA extraction kit provides a fast and simple method for the isolation of DNA from eucaryotic cells.
Purification requires neither phenol and chloroform extraction nor ethanol precipitation.
DNA purification is grounded on a selective binding of nucleic acids to a silica-based membrane in the presence of chaotropic salts. Non-nucleic acids elements do not bind to the membrane and will be removed by centrifugation. DNA is then purified of residual contaminants by passing the wash buffer twice through the membrane. DNA is then eluted in the
elution buffer, and is ready for use for enzymatic reactions such as restriction analyses or PCR.
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Introduction
The MBST DNA extraction kit provides a fast and simple method for the isolation of DNA from procaryotic cells.
Purification requires neither phenol and chloroform extraction nor ethanol precipitation.
DNA purification is grounded on a selective binding of nucleic acids to a silica-based membrane in the presence of chaotropic salts. Non-nucleic acids elements do not bind to the membrane and will be removed by centrifugation. DNA is then purified of residual contaminants by passing the wash buffer twice through the membrane. DNA is then eluted in the
elution buffer, and is ready for use for enzymatic reactions such as restriction analyses or PCR.
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